Welcome to the UConn Health Flow Cytometry Core
The UConn Health Flow Cytometry Facility provides flow
cytometric analysis and cell sorting services to all UConn
researchers as well as researchers at neighboring
institutions. The facility, located on the 6th floor of the
E building in room E6014, consists of a 900 square-foot lab
space, complete with fume hood, centrifuge, and sink space.
The facility currently has six instruments available for
cellular analysis and two cell sorters.
We house two four-color BD FACS Calibur instruments,
LSR II instruments, a MACSQuant10, and two BD FACS ARIA II
high speed cell sorters. Two of our LSR II instruments are
equipped with five lasers and are capable of 15 or 18 parameter
analysis. One of our LSR II instruments is equipped with
four lasers and is capable of 13 parameter analysis.
Our FACS ARIA II cell sorters are both equipped with five
lasers; one can analyze 15 parameters, and the other is
capable of 18 parameter analysis. Additionally, the 15
parameter FACS ARIA II is installed in a Baker Bioprotect
III safety cabinet, which facilitates analysis and sorting
of potentially biohazardous samples.
We also provide several computer workstations with flow
cytometry and cytometric bead array analysis software. These
include: FlowJo (Treestar Inc.), BD FACS DIVA (BD
Bioscience), Cell Quest Pro (BD Bioscience), ModFit LT
(Verity), and FCAP Array (BD Bioscience).
The facility is staffed Monday through Friday from 9 a.m.
to 7 p.m. After hours or weekend access is permitted for
trained UConn Health employees. Please contact us with any
questions regarding the facility, flow cytometry, or the
development of new applications.