Welcome to the UConn Health Center Flow Cytometry Core
The UConn Health Center Flow Cytometry Facility provides
flow cytometric analysis and cell sorting services to all
UConn researchers as well as researchers at neighboring
institutions. The facility, located on the 5th floor of the
L building in room L5058, consists of a 900 square-foot lab
space, complete with fume hood, centrifuge, and sink space.
The facility currently has five instruments available for
cellular analysis and two cell sorters.
We house 2 four-color BD FACS Calibur instruments, 3 BD
LSR II instruments, and 2 BD FACS ARIA II high speed cell
sorters. Two of our LSR II instruments are equipped with
four lasers and are capable of 13 parameter analysis. One of
our LSR II instruments is equipped with five lasers and is
capable of 18 parameter analysis.
Our FACS ARIA II cell sorters are both equipped with five
lasers; one can analyze 15 parameters, and the other is
capable of 18 parameter analysis. Additionally, the 15
parameter FACS ARIA II is installed in a Baker Bioprotect
III safety cabinet, which facilitates analysis and sorting
of potentially biohazardous samples.
We also provide several computer workstations with flow
cytometry and cytometric bead array analysis software. These
include: FlowJo (Treestar Inc.), BD FACS DIVA (BD
Bioscience), Cell Quest Pro (BD Bioscience), ModFit LT
(Verity), and FCAP Array (BD Bioscience).
The facility is staffed Monday through Friday from 9 a.m.
to 7 p.m. After hours or weekend access is permitted for
trained Health Center employees. Please contact us with any
questions regarding the facility, flow cytometry, or the
development of new applications.