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FACSAria Laser Configurations and Possible Fluorochrome
Usage
Becton-Dickinson FacsAria
|
Laser Line
|
Detector
|
Long Pass Filter
|
Band Pass
|
Fluor
|
|
488nm blue laser |
A |
755 |
780/60 |
PE-Cy7 |
|
B |
685 |
710/50 |
PerCP-Cy5.5, PI |
|
C |
670 |
675/20 |
PerCP |
|
D |
600 |
610/20 |
PE-Texas Red |
|
E |
550 |
575/25 |
PE |
|
F |
505 |
530/30 |
FITC, Alexa 488, GFP, CFSE |
|
G |
- |
480/10 |
SSC |
|
633nm red laser |
A |
755 |
780/60 |
APC-Cy7, APC-Alexa 750 |
|
B |
685 |
710/50 |
APC-Cy5.5, Alexa 700 |
|
C |
- |
660/20 |
APC |
|
405nm violet laser |
A |
502 |
530/30 |
Pacific Orange, Alexa 430, Am Cyan |
|
B |
- |
470/20 |
CFP, Pacific Blue |
|
C |
- |
- |
- |
|
355nm UV laser |
A |
635 |
670 LP |
Hoechst Red, Indo-1 (Blue) |
|
B |
- |
450/50 |
DAPI, Hoechst Blue |
|
C |
- |
- |
- |
|
561nm yellow/green laser |
A |
600 |
610/20 |
mCherry |
|
B |
570 |
582/15 |
DsRed |
|
C |
- |
- |
- |
|
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