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FACSCalibur Laser Configurations and Possible Fluorochrome
Usage
Becton-Dickinson FACSCalibur |
Laser Line
|
Fluorochromes
|
Excitation wavelength (nm)
|
Emission wavelength (nm)
|
|
488nm |
Fitc |
493 |
525 |
|
AlexaFluor 488 |
495 |
519 |
|
PE |
496, 565 |
575 |
|
PE-Texas Red |
496, 565 |
613 |
|
PE/Cy5 |
496, 565 |
670 |
|
PerCp |
482 |
675 |
|
PE/Cy5.5 |
496, 565 |
690 |
|
PerCP/Cy5.5 |
482 |
690 |
|
PE/Cy7 |
496, 565 |
774 |
|
Viability Probes: PI (Propidium iodide) |
325, 360, 488 |
620 |
|
7-AAD |
488 |
647 |
|
633nm |
APC |
645 |
660 |
|
Cy5 |
649 |
666 |
|
AlexaFluor 647 |
650 |
668 |
|
APC/CY5.5 |
650 |
690 |
|
APC/Cy7 |
650 |
774 |
|
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