LSR II Laser Configurations and Possible Fluorochrome
Usage (Same for LSR IIA and LSR IIB)
Becton-Dickinson LSR II
|
Laser Line
|
Detector
|
Long Pass Filter
|
Band Pass
|
Fluor
|
|
488nm blue laser |
A |
735 |
780/60 |
PE-Cy7, PE-Alexa 750 |
|
B |
685 |
695/30 |
PerCP-Cy5.5, PE-Cy5.5, 7AAD, PE-Alexa 700,
Alexa 680 |
|
C |
635 |
670/14 |
PerCP,
PE-Cy5, PE-Alexa 647, 7AAD, Fura-Low-Ca++ |
|
D |
600 |
610/20 |
PI, PE-Texas Red, PE-Alexa 610, PE-Alexa 594 |
|
E |
550 |
575/26 |
PE |
|
F |
505 |
530/30 |
Fitc, Alexa 488, YFP, GFP, CFSE, Calcein AM |
|
G |
- |
488/10 |
SSC |
|
633nm red laser |
A |
735 |
780/60 |
APC-H7,
APC-Cy7, APC-Alexa 750 |
|
B |
680 |
695/40 |
APC-Cy5.5; Alexa 700 with 710/20 filter |
|
C |
- |
660/20 |
APC, Alexa 647 |
|
405nm violet laser |
A |
505 |
585/42 |
Pacific Orange, Alexa 430 with 525/50 filter |
|
B |
- |
440/40 |
Alexa 405, CFP, Pacific Blue |
|
C |
- |
- |
- |
|
325nm (LSR IIA); 355nm (LSR IIB) UV Laser |
A |
505 |
525/50 |
Indo1 Blue |
|
B |
- |
440/40 |
DAPI,
Indo1 Violet, Hoechst 33342 |
|
C |
- |
- |
- |
|
